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Archive for the ‘Charles Brenner’ Category

FOEDRC Retreat Poster Winners: Po Hien Ear and Adam Rauckhorst

Thursday, January 5th, 2017

Congratulations to Postdoctoral Fellows Po Hien Ear (Brenner Lab) and Adam Rauckhorst (Taylor Lab) who were two of the three poster winners at the 2016 FOE Diabetes Research Center Retreat held on December 10, 2016, for their posters entitled “Nicotinamide riboside promotes maternal and neonatal health benefits” and “Novel mechanisms regulating mitochondrial glutamine metabolism,” respectively.

L to R: Adam Rauckhorst, Po Hien Ear, Yanhui Zhang, E. Dale Abel

2016 Biochemistry Newsletter now available

Friday, November 4th, 2016

2016_newslettercoverimageThe 2016 Biochemistry @ Iowa newsletter is hot off the presses and available for download. Alumni and friends should receive a hard copy in the mail this week. If you would like to be added to our mailing list, please send your contact information to biochem@uiowa.edu. While you are at it, feel free to send us your news and updates! Previous newsletters are also available online.

Brenner lab’s clinical trial on the effects of NR in humans shows the compound safely increases levels of important cell metabolite

Thursday, October 13th, 2016

Brenner_Charles_2015_reducedIn a study published on October 10 in Nature Communications, Dr. Charles Brenner, Roy J. Carver Chair of Biochemistry, in collaboration with visiting Levitt Professor, Dr. Marie Migaud, shows nicotinamide riboside (NR) increases levels of a cell metabolite that is critical for cellular energy production and protection against stress and DNA damage in humans. This was the first controlled clinical trial of the effects of NR in humans. “This trial shows that oral NR safely boosts human NAD+ metabolism,” Brenner says. “We are excited because everything we are learning from animal systems indicates that the effectiveness of NR depends on preserving and/or boosting NAD+ and related compounds in the face of metabolic stresses. Because the levels of supplementation in mice that produce beneficial effects are achievable in people, it appears that health benefits of NR will be translatable to humans safely.” This study was also featured on IowaNow.

Brenner lab treats type 2 diabetic neuropathy with nicotinamide riboside

Tuesday, May 31st, 2016

In the new study, published May 27 in the journal Scientific Reports, Dr. Charles Brenner, together with Randy Kardon, MD, PhD, and Mark Yorek, PhD, who are jointly affiliated with University of Iowa Health Care and the Iowa City VA Health System, and Samuel Trammell (2016 PhD, Brenner laboratory), tested the effects of NR supplementation on mouse models of prediabetes and type 2 diabetes. Read more about their study here: http://www.eurekalert.org/pub_releases/2016-05/uoih-vnr052616.php

2015 Publications

Wednesday, March 16th, 2016

The Department’s 2015 publications are now in print and mostly available online.

E. Dale Abel

Jaishy B, Zhang Q, Chung HS, Riehle C, Soto J, Jenkins S, Abel P, Cowart LA, Van Eyk JE, Abel ED. (2015) Lipid-induced NOX2 activation inhibits autophagic flux by impairing lysosomal enzyme activity. J Lipid Res. 56(3):546-61. DOI: 10.1194/jlr.M055152. PMID: 25529920

Winkler EA, Nishida Y, Sagare AP, Rege SV, Bell RD, Perlmutter D, Sengillo JD, Hillman S, Kong P, Nelson AR, Sullivan JS, Zhao Z, Meiselman HJ, Wenby RB, Soto J, Abel ED, Makshanoff J, Zuniga E, De Vivo DC, Zlokovic BV. (2015) GLUT1 reductions exacerbate Alzheimer’s disease vasculo-neuronal dysfunction and degeneration. Nat Neurosci. 18(4):521-30. DOI: 10.1038/nn.3966. PMID: 25730668

Koentges C, König A, Pfeil K, Hölscher ME, Schnick T, Wende AR, Schrepper A, Cimolai MC, Kersting S, Hoffmann MM, Asal J, Osterholt M, Odening KE, Doenst T, Hein L, Abel ED, Bode C, Bugger H. (2015) Myocardial mitochondrial dysfunction in mice lacking adiponectin receptor 1. Basic Res Cardiol. 110(4):37. DOI: 10.1007/s00395-015-0495-4. PMID: 25982881

Ilkun O, Wilde N, Tuinei J, Pires KM, Zhu Y, Bugger H, Soto J, Wayment B, Olsen C, Litwin SE, Abel ED. (2015) Antioxidant treatment normalizes mitochondrial energetics and myocardial insulin sensitivity independently of changes in systemic metabolic homeostasis in a mouse model of the metabolic syndrome. J Mol Cell Cardiol. 85:104-16. DOI: 10.1016/j.yjmcc.2015.05.012. PMID: 26004364

Rasmussen TP, Wu Y, Joiner ML, Koval OM, Wilson NR, Luczak ED, Wang Q, Chen B, Gao Z, Zhu Z, Wagner BA, Soto J, McCormick ML, Kutschke W, Weiss RM, Yu L, Boudreau RL, Abel ED, Zhan F, Spitz DR, Buettner GR, Song LS, Zingman LV, Anderson ME. (2015) Inhibition of MCU forces extramitochondrial adaptations governing physiological and pathological stress responses in heart. Proc Natl Acad Sci USA. 112(29):9129-34. DOI: 10.1073/pnas.1504705112. PMID: 26153425

Liao X, Zhang R, Lu Y, Prosdocimo DA, Sangwung P, Zhang L, Zhou G, Anand P, Lai L, Leone TC, Fujioka H, Ye F, Rosca MG, Hoppel CL, Schulze PC, Abel ED, Stamler JS, Kelly DP, Jain MK. (2015) Kruppel-like factor 4 is critical for transcriptional control of cardiac mitochondrial homeostasis. J Clin Invest. 125(9):3461-76. DOI: 10.1172/JCI79964. PMID: 26241060

Bharath LP, Ruan T, Li Y, Ravindran A, Wan X, Nhan JK, Walker ML, Deeter L, Goodrich R, Johnson E, Munday D, Mueller R, Kunz D, Jones D, Reese V, Summers SA, Babu PV, Holland WL, Zhang QJ, Abel ED, Symons JD. (2015) Ceramide-Initiated Protein Phosphatase 2A Activation Contributes to Arterial Dysfunction In Vivo. Diabetes. 64(11):3914-26. DOI: 10.2337/db15-0244. PMID: 26253611

Ho PC, Bihuniak JD, Macintyre AN, Staron M, Liu X, Amezquita R, Tsui YC, Cui G, Micevic G, Perales JC, Kleinstein SH, Abel ED, Insogna KL, Feske S, Locasale JW, Bosenberg MW, Rathmell JC, Kaech SM. (2015) Phosphoenolpyruvate Is a Metabolic Checkpoint of Anti-tumor T Cell Responses. Cell. 162(6):1217-28. DOI: 10.1016/j.cell.2015.08.012. PMID: 26321681

Ock S, Lee WS, Ahn J, Kim HM, Kang H, Kim HS, Jo D, Abel ED, Lee TJ, Kim J. (2015) Deletion of IGF-1 Receptors in Cardiomyocytes Attenuates Cardiac Aging in Male Mice. Endocrinology. 157(1):336-45. DOI: 10.1210/en.2015-1709. PMID: 26469138

Ni R, Zheng D, Xiong S, Hill DJ, Sun T, Gardiner RB, Fan GC, Lu Y, Abel ED, Greer PA, Peng T. (2015) Mitochondrial Calpain-1 Disrupts ATP Synthase and Induces Superoxide Generation in Type 1 Diabetic Hearts: A Novel Mechanism Contributing to Diabetic Cardiomyopathy. Diabetes. 65(1):255-68. DOI: 10.2337/db15-0963. PMID: 26470784

Noh J, Wende AR, Olsen CD, Kim B, Bevins J, Zhu Y, Zhang QJ, Riehle C, Abel ED.( 2015) Phosphoinositide dependent protein kinase 1 is required for exercise-induced cardiac hypertrophy but not the associated mitochondrial adaptations. J Mol Cell Cardiol. 89(Pt B):297-305. DOI: 10.1016/j.yjmcc.2015.10.015. PMID: 26476238

Sheila A. Baker

Pan Y, Laird JG, Yamaguchi DM, Baker SA. (2015) An N-Terminal ER Export Signal Facilitates the Plasma Membrane Targeting of HCN1 Channels in Photoreceptors. Invest Ophthalmol Vis Sci. 56(6):3514-21. DOI: 10.1167/iovs.15-16902. PMID: 26030105

Laird JG, Pan Y, Modestou M, Yamaguchi DM, Song H, Sokolov M, Baker SA. (2015) Identification of a VxP Targeting Signal in the Flagellar Na(+) /K(+) -ATPase. Traffic. 16(12):1239-53. DOI: 10.1111/tra.12332. PMID: 26373354

Cao Y, Sarria I, Fehlhaber KE, Kamasawa N, Orlandi C, James KN, Hazen J, Gardner MR, Farzan M, Lee A, Baker SA, Baldwin K, Sampath AP, Martemyanov KA. (2015) Mechanism for selective synaptic wiring of rod photoreceptors into the retinal circuitry and its role in vision. Neuron. 87(6):1248-60. PMID: 26402607

Charles M. Brenner

Trammell SA, Brenner C. (2015) NNMT: A Bad Actor in Fat Makes Good in Liver. Cell Metab. 22(2):200-1. DOI: 10.1016/j.cmet.2015.07.017. PMID: 26244929

Mei SC, Brenner C. (2015) Calorie restriction-mediated replicative lifespan extension in yeast is non-cell autonomous. PLoS Biol. 13(1):e1002048. DOI: 10.1371/journal.pbio.1002048. PMID: 25633578

John M. Dagle

Waleh N, Barrette AM, Dagle JM, Momany A, Jin C, Hills NK, Shelton EL, Reese J, Clyman RI. (2015) Effects of Advancing Gestation and Non-Caucasian Race on Ductus Arteriosus Gene Expression. J Pediatr. 167(5):1033-1041.e2. DOI: 10.1016/j.jpeds.2015.07.011. PMID: 26265282

Sampath V, Helbling D, Menden H, Dimmock D, Mulrooney NP, Murray JC, Dagle JM, Garland JS. (2015) Necrotizing Enterocolitis is Not Associated with Sequence Variants in Anti-Oxidant Response Genes in Premature Infants.J Pediatr Gastroenterol Nutr. PMID: 26426434

Ryckman KK, Berberich SL, Dagle JM. (2015) Predicting gestational age by the use of neonatal metabolic markers. Am J Obstet Gynecol. pii: S0002-9378(15)02401-1. DOI: 10.1016/j.ajog.2015.11.028. PMID: 26645954

Kaluarachchi DC, Momany AM, Busch TD, Gimenez LG, Saleme C, Cosentino V, Christensen K, Dagle JM, Ryckman KK, Murray JC. (2015) Polymorphisms in NR5A2, gene encoding liver receptor homolog-1 are associated with preterm birth. Pediatr Res. DOI: 10.1038/pr.2016.7. PMID: 26761123

Brandon S. Davies

Beigneux AP, Fong LG, Bensadoun A, Davies BS, Oberer M, Gårdsvoll H, Ploug M, Young SG. (2015) GPIHBP1 missense mutations often cause multimerization of GPIHBP1 and thereby prevent lipoprotein lipase binding. Circ Res. 116(4):624-32. DOI: 10.1161/CIRCRESAHA.116.305085. PMID: 25387803

Chi X, Shetty SK, Shows HW, Hjelmaas AJ, Malcolm EK, Davies BS. (2015) Angiopoietin-like 4 Modifies the Interactions between Lipoprotein Lipase and Its Endothelial Cell Transporter GPIHBP1.J Biol Chem. 290(19):11865-77. DOI: 10.1074/jbc.M114.623769. PMID: 25809481

Kris A. DeMali

Jannie K, Ellerbroek SM, Zhou DW, Chen S, Crompton DJ, Garcia AJ, DeMali KA (2015) Vinculin-dependent actin bundling regulates cell migration and traction forces. Biochem. J. 465 (3) 383-393. DOI: 10.1042/BJ20140872. PMID: 25358683

Adrian H. Elcock

Li S, Elcock AH. (2015) Residue-Specific Force Field (RSFF2) Improves the Modeling of Conformational Behavior of Peptides and Proteins. J Phys Chem Lett. 6(11):2127-33. DOI: 10.1021/acs.jpclett.5b00654. PMID: 26266514

Cressiot B, Braselmann E, Oukhaled A, Elcock AH, Pelta J, Clark PL. (2015) Dynamics and Energy Contributions for Transport of Unfolded Pertactin through a Protein Nanopore. ACS Nano. 9(9):9050-61. DOI: 10.1021/acsnano.5b03053. PMID: 26302243

Brown RF, Andrews CT, Elcock AH. (2015) Stacking Free Energies of All DNA and RNA Nucleoside Pairs and Dinucleoside-Monophosphates Computed Using Recently Revised AMBER Parameters and Compared with Experiment. J Chem Theory Comput. 11(5):2315-28. DOI: 10.1021/ct501170h.PMID:26574427

Frembgen-Kesner T, Andrews CT, Li S, Ngo NA, Shubert SA, Jain A, Olayiwola OJ, Weishaar MR, Elcock AH. (2015) Parametrization of Backbone Flexibility in a Coarse-Grained Force Field for Proteins (COFFDROP) Derived from All-Atom Explicit-Solvent Molecular Dynamics Simulations of All Possible Two-Residue Peptides. J Chem Theory Comput. 11(5):2341-54. DOI: 10.1021/acs.jctc.5b00038. PMID: 26574429

Li S, Andrews CT, Frembgen-Kesner T, Miller MS, Siemonsma SL, Collingsworth TD, Rockafellow IT, Ngo NA, Campbell BA, Brown RF, Guo C, Schrodt M, Liu YT, Elcock AH. (2015) Molecular Dynamics Simulations of 441 Two-Residue Peptides in Aqueous Solution: Conformational Preferences and Neighboring Residue Effects with the Amber ff99SB-ildn-NMR Force Field. J Chem Theory Comput. 11(3):1315-29. DOI: 10.1021/ct5010966. PMID: 26579777

Schrodt MV, Andrews CT, Elcock AH. (2015) Large-Scale Analysis of 48 DNA and 48 RNA Tetranucleotides Studied by 1 μs Explicit-Solvent Molecular Dynamics Simulations. J Chem Theory Comput. 11(12):5906-17. DOI: 10.1021/acs.jctc.5b00899. PMID: 26580891

Lokesh Gakhar

Yadav RP, Majumder A, Gakhar L, Artemyev NO. (2015) Extended conformation of the proline-rich domain of human aryl hydrocarbon receptor-interacting protein-like 1: implications for retina disease. J Neurochem. 135(1):165-75. DOI: 10.1111/jnc.13223. PMID: 26139345

Wert KJ, Bassuk AG, Wu WH, Gakhar L, Coglan D, Mahajan M, Wu S, Yang J, Lin CS, Tsang SH, Mahajan VB. (2015) CAPN5 mutation in hereditary uveitis: the R243L mutation increases calpain catalytic activity and triggers intraocular inflammation in a mouse model. Hum Mol Genet. 24(16):4584-98. DOI: 10.1093/hmg/ddv189. PMID: 25994508

Bassuk AG, Yeh S, Wu S, Martin DF, Tsang SH, Gakhar L, Mahajan VB. (2015) Structural modeling of a novel CAPN5 mutation that causes uveitis and neovascular retinal detachment. PLoS One. 10(4):e0122352. DOI: 10.1371/journal.pone.0122352. PMID: 25856303

Feldkamp MD, Gakhar L, Pandey N, Shea MA. (2015) Opposing orientations of the anti-psychotic drug trifluoperazine selected by alternate conformations of M144 in calmodulin. Proteins. 83(5):989-96. DOI: 10.1002/prot.24781. PMID: 25694384

Cho C, Chande A, Gakhar L, Bakaletz LO, Jurcisek JA, Ketterer M, Shao J, Gotoh K, Foster E, Hunt J, O’Brien E, Apicella MA. (2015) Role of the nuclease of nontypeable Haemophilus influenzae in dispersal of organisms from biofilms. Infect Immun. 83(3):950-7. DOI: 10.1128/IAI.02601-14. PMID: 25547799

Doron D, Stojković V, Gakhar L, Vardi-Kilshtain A, Kohen A, Major DT. (2015) Free energy simulations of active-site mutants of dihydrofolate reductase. J Phys Chem B. 119(3):906-16. doi: 10.1021/jp5059963. PMID: 25382260

Pamela K. Geyer

Barton LJ, Soshnev AA, Geyer PK. (2015) Networking in the nucleus: a spotlight on LEM-domain proteins. Curr Opin Cell Biol. 34:1-8. DOI: 10.1016/j.ceb.2015.03.005.PMID: 25863918

Catherine A. Musselman

Tong Q, Cui G, Botuyan MV, Rothbart SB, Hayashi R, Musselman CA, Singh N, Appella E, Strahl BD, Mer G, Kutateladze TG. (2015) Structural plasticity of methyllysine recognition by the tandem tudor domain of 53BP1. Structure. 23(2):312-21. DOI: 10.1016/j.str.2014.11.013. PMID: 25579814

Andrew W. Norris

Uc A, Olivier AK, Griffin MA, Meyerholz DK, Yao J, Abu-El-Haija M, Buchanan KM, Vanegas Calderón OG, Abu-El-Haija M, Pezzulo AA, Reznikov LR, Hoegger MJ, Rector MV, Ostedgaard LS, Taft PJ, Gansemer ND, Ludwig PS, Hornick EE, Stoltz DA, Ode KL, Welsh MJ, Engelhardt JF, Norris AW. (2015) Glycaemic regulation and insulin secretion are abnormal in cystic fibrosis pigs despite sparing of islet cell mass. Clin Sci (Lond). 128(2):131-42. DOI: 10.1042/CS20140059. PMID: 25142104

Morgan DA, McDaniel LN, Yin T, Khan M, Jiang J, Acevedo MR, Walsh SA, Ponto LL, Norris AW, Lutter M, Rahmouni K, Cui H. (2015) Regulation of glucose tolerance and sympathetic activity by MC4R signaling in the lateral hypothalamus. Diabetes. 64(6):1976-87. DOI: 10.2337/db14-1257. PMID: 25605803

Gordon EE, Reinking BE, Hu S, Yao J, Kua KL, Younes AK, Wang C, Segar JL, Norris AW. (2015) Maternal Hyperglycemia Directly and Rapidly Induces Cardiac Septal Overgrowth in Fetal Rats. J Diabetes Res. 2015:479565. DOI: 10.1155/2015/479565. PMID: 26064981

Gray LR, Sultana MR, Rauckhorst AJ, Oonthonpan L, Tompkins SC, Sharma A, Fu X, Miao R, Pewa AD, Brown KS, Lane EE, Dohlman A, Zepeda-Orozco D, Xie J, Rutter J, Norris AW, Cox JE, Burgess SC, Potthoff MJ, Taylor EB. (2015) Hepatic Mitochondrial Pyruvate Carrier 1 Is Required for Efficient Regulation of Gluconeogenesis and Whole-Body Glucose Homeostasis. Cell Metab. 22(4):669-81. DOI: 10.1016/j.cmet.2015.07.027. PMID: 26344103

Sawatzke AB, Norris AW, Spyropoulos F, Walsh SA, Acevedo MR, Hu S, Yao J, Wang C, Sunderland JJ, Boles Ponto LL. (2015) PET/CT imaging reveals unrivaled placental avidity for glucose compared to other tissues. Placenta. 36(2):115-20. DOI: 10.1016/j.placenta.2014.12.009. PMID: 25555498

Bryce V. Plapp

Plapp BV, Leidal KG, Murch BP, Green DW. (2015) Contribution of liver alcohol dehydrogenase to metabolism of alcohols in rats. Chem Biol Interact. 234:85-95. DOI: 10.1016/j.cbi.2014.12.040. PMID: 25641189

David H. Price

Nilson KA, Guo J, Turek ME, Brogie JE, Delaney E, Luse DS, Price DH. (2015) THZ1 Reveals Roles for Cdk7 in Co-transcriptional Capping and Pausing. Mol Cell. 59(4):576-87. DOI: 10.1016/j.molcel.2015.06.032. PMID: 26257281

Miles A. Pufall

Fontana MF, Baccarella A, Pancholi N, Pufall MA, Herbert DR, Kim CC. (2015) JUNB is a key transcriptional modulator of macrophage activation. J Immunol. 194(1):177-86.DOI: 10.4049/jimmunol.1401595. PMID: 25472994

Sasse SK, Zuo Z, Kadiyala V, Zhang L, Pufall MA, Jain MK, Phang TL, Stormo GD, Gerber AN. (2015) Response Element Composition Governs Correlations between Binding Site Affinity and Transcription in Glucocorticoid Receptor Feed-forward Loops. J Biol Chem. 290(32):19756-69. DOI: 10.1074/jbc.M115.668558. PMID: 26088140

Pufall MA. (2015) Glucocorticoids and Cancer. Adv Exp Med Biol. 872:315-33. DOI: 10.1007/978-1-4939-2895-8_14. PMID: 26216001

Peter A. Rubenstein

Rubenstein PA, Wen KK. (2015) Mutant vascular actin is a TAAD misbehaving. Proc Natl Acad Sci USA. 112(31):9500-1. DOI: 10.1073/pnas.1512086112. PMID: 26204915

Michael J. Schnieders

Bu F, Borsa NG, Jones MB, Takanami E, Nishimura C, Hauer JJ, Azaiez H, Black-Ziegelbein EA, Meyer NC, Kolbe DL, Li Y, Frees K, Schnieders MJ, Thomas C, Nester C, Smith RJ. 92015) High-Throughput Genetic Testing for Thrombotic Microangiopathies and C3 Glomerulopathies. J Am Soc Nephrol. pii: ASN.2015040385. PMID:26283675

LuCore SD, Litman JM, Powers KT, Gao S, Lynn AM, Tollefson WT, Fenn TD, Washington MT, Schnieders MJ. (2015) Dead-End Elimination with a Polarizable Force Field Repacks PCNA Structures. Biophys J. 109(4):816-26. DOI: 10.1016/j.bpj.2015.06.062. PMID: 26287633

Booth KT, Azaiez H, Kahrizi K, Simpson AC, Tollefson WT, Sloan CM, Meyer NC, Babanejad M, Ardalani F, Arzhangi S, Schnieders MJ, Najmabadi H, Smith RJ. (2015) PDZD7 and hearing loss: More than just a modifier. Am J Med Genet A. 167(12):2957-65. DOI: 10.1002/ajmg.a.37274. PMID: 26416264

DeLuca AP, Whitmore SS, Barnes J, Sharma TP, Westfall TA, Scott CA, Weed MC, Wiley JS, Wiley LA, Johnston RM, Schnieders MJ, Lentz SR, Tucker BA, Mullins RF, Scheetz TE, Stone EM, Slusarski DC. (2015) Hypomorphic mutations in TRNT1 cause retinitis pigmentosa with erythrocytic microcytosis. Hum Mol Genet. 25(1):44-56. DOI: 10.1093/hmg/ddv446. PMID: 26494905

Lagardère L, Lipparini F, Stamm B, Schnieders MJ, Cances E, Ren P, Maday Y, Piquemal J. (2015) Scalable Evaluation of Polarization Energy and Associated Forces in Polarizable Molecular Dynamics: II. Towards Massively Parallel Computations Using Smooth Particle Mesh Ewald. Journal of Chemical Theory and Computation. 11(6):2589–2599.

Debra A. Schwinn

Levine AS, Alpern RJ, Andrews NC, Antman K, Balser JR, Berg JM, Davis PB, Fitz JG, Golden RN, Goldman L, Jameson JL, Lee VS, Polonsky KS, Rappley MD, Reece EA, Rothman PB, Schwinn DA, Shapiro LJ, Spiegel AM. (2015) Research in academic medical centers: two threats to sustainable support. Sci Transl Med. 7(289):289fs22. DOI: 10.1126/scitranslmed.aac5200. PMID: 26019216

Morris DP, Lei B, Longo LD, Bomsztyk K, Schwinn DA, Michelotti GA. (2015) Temporal Dissection of Rate Limiting Transcriptional Events Using Pol II ChIP and RNA Analysis of Adrenergic Stress Gene Activation. PLoS One. 10(8):e0134442. DOI: 10.1371/journal.pone.0134442. PMID: 26244980

Gradinaru I, Babaeva E, Schwinn DA, Oganesian A. (2015) Alpha1a-Adrenoceptor Genetic Variant Triggers Vascular Smooth Muscle Cell Hyperproliferation and Agonist Induced Hypertrophy via EGFR Transactivation Pathway. PLoS One. 10(11):e0142787. DOI: 10.1371/journal.pone.0142787. PMID: 26571308

Madeline A. Shea

Feldkamp MD, Gakhar L, Pandey N, Shea MA. (2015) Opposing orientations of the anti-psychotic drug trifluoperazine selected by alternate conformations of M144 in calmodulin. Proteins. 83(5):989-96. DOI: 10.1002/prot.24781. PMID: 25694384

M. Ashley Spies

Dean SF, Whalen KL, Spies MA. (2015) Biosynthesis of a Novel Glutamate Racemase Containing a Site-Specific 7-Hydroxycoumarin Amino Acid: Enzyme-Ligand Promiscuity Revealed at the Atomistic Level. ACS Cent Sci. 1(7):364-373. PMID: 26539562

Marsden AE, King JM, Spies MA, Kim OK, Yahr TL. (2015) Inhibition of Pseudomonas aeruginosa ExsA DNA-Binding Activity by N-Hydroxybenzimidazoles. Antimicrob Agents Chemother. 60(2):766-76. DOI: 10.1128/AAC.02242-15. PMID: 26574012

Maria Spies

Spies M, Fishel R. (2015) Mismatch repair during homologous and homeologous recombination. Cold Spring Harb Perspect Biol. 7(3):a022657. DOI: 10.1101/cshperspect.a022657. PMID: 25731766

Earle Stellwagen

Stellwagen E, Dong Q, Stellwagen NC. (2015) Flanking A·T basepairs destabilize the B(∗) conformation of DNA A-tracts. Biophys J. 108(9):2291-9. DOI: 10.1016/j.bpj.2015.01.044. PMID: 25954886

Stellwagen E, Stellwagen NC. (2015) Electrostatic coupling between DNA and its counterions modulates the observed translational diffusion coefficients. Anal Chem. 87(17):9042-6. DOI: 10.1021/acs.analchem.5b02230. PMID: 26218420

Nancy C. Stellwagen

Stellwagen E, Dong Q, Stellwagen NC. (2015) Flanking A·T basepairs destabilize the B(∗) conformation of DNA A-tracts. Biophys J. 108(9):2291-9. DOI: 10.1016/j.bpj.2015.01.044. PMID: 25954886

Stellwagen E, Stellwagen NC. (2015) Electrostatic coupling between DNA and its counterions modulates the observed translational diffusion coefficients. Anal Chem. 87(17):9042-6. DOI: 10.1021/acs.analchem.5b02230. PMID: 26218420

Eric B. Taylor

Gray LR, Sultana MR, Rauckhorst AJ, Oonthonpan L, Tompkins SC, Sharma A, Fu X, Miao R, Pewa AD, Brown KS, Lane EE, Dohlman A, Zepeda-Orozco D, Xie J, Rutter J, Norris AW, Cox JE, Burgess SC, Potthoff MJ, Taylor EB. (2015) Hepatic Mitochondrial Pyruvate Carrier 1 Is Required for Efficient Regulation of Gluconeogenesis and Whole-Body Glucose Homeostasis. Cell Metab. 22(4):669-81. DOI: 10.1016/j.cmet.2015.07.027. PMID: 26344103

Lori L. Wallrath

Bohnekamp J, Cryderman DE, Paululat A, Baccam GC, Wallrath LL, Magin TM. (2015) A Drosophila Model of Epidermolysis Bullosa Simplex. J Invest Dermatol. 135(8):2031-9. DOI: 10.1038/jid.2015.129. PMID: 25830653

Dialynas G, Shrestha OK, Ponce JM, Zwerger M, Thiemann DA, Young GH, Moore SA, Yu L, Lammerding J, Wallrath LL. (2015) Myopathic lamin mutations cause reductive stress and activate the nrf2/keap-1 pathway. PLoS Genet. 11(5):e1005231. DOI: 10.1371/journal.pgen.1005231. PMID: 25996830

Bohnekamp J, Cryderman DE, Thiemann DA, Magin TM, Wallrath LL. (2015) Using Drosophila for Studies of Intermediate Filaments. Methods Enzymol. 568:707-26. DOI: 10.1016/bs.mie.2015.08.028. PMID: 26795490

M. Todd Washington

Tsutakawa SE, Yan C, Xu X, Weinacht CP, Freudenthal BD, Yang K, Zhuang Z, Washington MT, Tainer JA, Ivanov I. (2015) Structurally distinct ubiquitin- and sumo-modified PCNA: implications for their distinct roles in the DNA damage response. Structure. 23(4):724-33. DOI: 10.1016/j.str.2015.02.008. PMID: 25773143

LuCore SD, Litman JM, Powers KT, Gao S, Lynn AM, Tollefson WT, Fenn TD, Washington MT, Schnieders MJ. (2015) Dead-End Elimination with a Polarizable Force Field Repacks PCNA Structures. Biophys J. 109(4):816-26. DOI: 10.1016/j.bpj.2015.06.062. PMID: 26287633

Ronald J. Weigel

Borcherding N, Kusner D, Kolb R, Xie Q, Li W, Yuan F, Velez G, Askeland R, Weigel RJ, Zhang W. (2015) Paracrine WNT5A Signaling Inhibits Expansion of Tumor-Initiating Cells. Cancer Res. 75(10):1972-82. DOI: 10.1158/0008-5472.CAN-14-2761. PMID: 25769722

Lizarraga I, Schroeder MC, Weigel RJ, Thomas A. (2015) Surgical Management of Breast Cancer in 2010-2011 SEER Registries by Hormone and HER2 Receptor Status. Ann Surg Oncol. 22 Suppl 3:566-72. DOI: 10.1245/s10434-015-4591-3. PMID: 25956579

Spanheimer PM, Lorenzen AW, De Andrade JP, Kulak MV, Carr JC, Woodfield GW, Sugg SL, Weigel RJ. (2015) Receptor Tyrosine Kinase Expression Predicts Response to Sunitinib in Breast Cancer. Ann Surg Oncol. 22(13):4287-94. DOI: 10.1245/s10434-015-4597-x. PMID: 25971960

Weigel RJ. (2015) 2015 Presidential Address–Society of Surgical Oncology: The Next 75 Years. Ann Surg Oncol. 22(8):2455-61. DOI: 10.1245/s10434-015-4638-5. PMID: 26033181

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Meng Wu

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Wongrakpanich A, Wu M, Salem AK. (2015) Correlating intracellular nonviral polyplex localization with transfection efficiency using high-content screening. Biotechnol Prog. 31(6):1685-92. DOI: 10.1002/btpr.2146. PMID: 26193826

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Zhu Y, Lu Y, Qu C, Miller M, Tian J, Thakur DP, Zhu J, Deng Z, Hu X, Wu M, McManus OB, Li M, Hong X, Zhu MX, Luo HR. (2015) Identification and optimization of 2-aminobenzimidazole derivatives as novel inhibitors of TRPC4 and TRPC5 channels. Br J Pharmacol. 172(14):3495-509. DOI: 10.1111/bph.13140. PMID: 25816897

Li J, Crowley ST, Duskey J, Khargharia S, Wu M, Rice KG. (2015) Miniaturization of gene transfection assays in 384- and 1536-well microplates. Anal Biochem. 470:14-21. DOI: 10.1016/j.ab.2014.10.001. PMID: 25448623

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Liping Yu

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Samuel Trammell successfully defends PhD thesis

Tuesday, February 23rd, 2016

Oonthonpan and TrammellSamuel Trammell, a genetics PhD Student mentored by Dr. Charles Brenner, successfully defended his thesis on February 22, 2016, entitled “Novel NAD+ metabolomic technologies and their applications to nicotinamide riboside interventions.” Sam has accepted a position as a postdoctoral fellow at the University of Copenhagen with Dr. Matthew Gillium working on lipid metabolism in diabetes.

Congratulations, Dr. Trammell!

Dr. Brenner interviewed in Anti-Aging Medicine, the magazine of the Japanese Society of Anti-Aging Medicine

Wednesday, December 9th, 2015

Brenner_Charles_2015_reducedDr. Charles Brenner was recently interviewed by Dr. Kazuo Tzubota, President of the Japanese Society of Anti-Aging Medicine, on the topic of NAD, nicotinamide riboside supplementation and aging. The interview was published in the Society’s magazine Anti-Aging Medicine, read the full interview here.

2015 Biochemistry Newsletter now available

Tuesday, October 20th, 2015

Cover_2015_newsletterThe 2015 Biochemistry @ Iowa newsletter is hot off the presses and available for download. Alumni and friends should receive a hard copy in the mail this week. If you would like to be added to our mailing list, please send your contact information to biochem@uiowa.edu. While you are at it, feel free to send us your news and updates! Previous newsletters are also available online.

Brenner receives ASBMB Award for Exemplary Contributions to Education

Thursday, August 20th, 2015

Brenner_Charles_2015_reduced

Dr. Charles Brenner, the Roy J. Carver Chair and Head of Biochemistry at the University of Iowa, has been selected to receive the 2016 ASBMB Award for Exemplary Contributions to Education, given annually to a scientist who encourages effective teaching and learning of biochemistry and molecular biology through his own teaching, leadership in education, writing, educational research, mentoring or public enlightenment.

Late in 2011, Dr. Brenner observed that the new MCAT, which was rolled out in April of 2015, would test core concepts in biochemistry for the first time and that this would have ramifications well beyond MCAT test takers. In his writings on this subject, Dr. Brenner pointed out that US colleges and universities enroll approximately 3 million new US  college freshmen per year and that as many as 500,000 begin the premedical sequence by enrolling in general chemistry. However, only about 165,000 of these students go on to take organic chemistry and 45,000 of these students apply annually to medical school for approximately 19,000 seats. Thus, the courses that are designed for premedical students actually engage a huge swath of students that end up doing something else. Thoughtful revision of the premedical curriculum has the potential to improve general education, the preparation of future researchers, educators and business people, and the preparation of health professionals (Brenner & Ringe, ASBMB Today , p. 12, 2012, Brenner, Biochem Mol Biol Ed, p 1, 2013 & Brenner, J Chem Ed, p. 807, 2013).

Specifically, in premedical curricular guidelines that were initially published in ASBMB Today, Dr. Brenner and Dr. Dagmar Ringe of Brandeis University began by recommending that the typically mandated year of biology provide a strong foundation in molecular genetics and biological information flow. Second, they recommended that chemistry courses move from the classical synthetic, organohalide orientation to one which emphasizes the reactivity of functionalized carbon and biomolecules in aqueous solutions. Third, they emphasized that a laboratory course is essential but that it could be offered in any department or discipline, so long as it includes instruction in data analysis. Finally, they wrote that premedical students take at least one semester and preferably two semesters of biochemistry in order to have the grounding to work in modern medicine.

Nationally, these recommendations appear to be having some impact. Where biochemistry courses are available, enrollment has burgeoned and in small colleges, faculty in chemistry and biology departments have begun offering biochemistry. At the University of Nebraska, Lincoln, the two semester biochemistry series is the expectation for pre-health students. At many other colleges and universities, chemistry and biochemistry coursework is being developed that will create a more continuous pedagogical basis for understanding molecular science.

In her letter of nomination for Dr. Brenner, Dean Debra Schwinn wrote “Dr. Brenner is powerfully and constructively engaged locally and nationally in medical education. At his urging, the Carver College of Medicine made undergraduate biochemistry a formal premedical requirement and with his effective influence, there has been a substantial preservation of biochemistry in the first semester of our new integrated medical curriculum. Indeed, educational leaders here at Iowa all read and appreciated his involvement in the national dialog on the need to reemphasize molecular science in the education of current and future medical students (Kennelly, et al., Acad Med 88, p. 1405, 2013).”

Dr. Brenner will present a plenary symposium lecture entitled “Biochemistry and molecular biology education in a transforming academy and a molecular world” at the ASBMB Annual meeting, April 2-6, 2016 in San Diego, CA.

 

Marie Migaud receives Levitt Endowed Visiting Professorship

Wednesday, July 1st, 2015

MigaudDr. Marie Migaud, of Queen’s University Belfast, was awarded the Helen C. Levitt Endowed Visiting Professorship in the University of Iowa Carver College of Medicine to perform research in collaboration with the laboratory of Dr. Charles Brenner. Dr. Migaud is a synthetic organic chemist who specializes in nucleosides and nucleotides. She has developed new synthetic technologies that have allowed her to produce novel analogs of nicotinamide riboside and, in collaboration with Dr. Brenner, she aims to develop a chemical biology toolkit to probe the intricacies of NAD metabolism in vertebrate systems. She will begin her 1 year visiting professorship in November 2015.