Link: University of Iowa

Tingting Duan won second place in the Art in Science Competition at the Iowa Microscopy Society

October 20th, 2016 by Miranda Nielson


Tingting Duan, a Biochemistry PhD student mentored by Dr. Pamela Geyer, was awarded second place in the Art in Science Competition by voters attending the Iowa Microscopy Society Fall Symposium. Her image of a tetis carpet “[drew] out beauty on an extremely small scale.” The goal of the Iowa Art in Science Contest is to recognize the combination of outstanding scientific discovery and artistic appeal inherent to microscopy research.

Brenner lab’s clinical trial on the effects of NR in humans shows the compound safely increases levels of important cell metabolite

October 13th, 2016 by Maren Rogers

Brenner_Charles_2015_reducedIn a study published on October 10 in Nature Communications, Dr. Charles Brenner, Roy J. Carver Chair of Biochemistry, in collaboration with visiting Levitt Professor, Dr. Marie Migaud, shows nicotinamide riboside (NR) increases levels of a cell metabolite that is critical for cellular energy production and protection against stress and DNA damage in humans. This was the first controlled clinical trial of the effects of NR in humans. “This trial shows that oral NR safely boosts human NAD+ metabolism,” Brenner says. “We are excited because everything we are learning from animal systems indicates that the effectiveness of NR depends on preserving and/or boosting NAD+ and related compounds in the face of metabolic stresses. Because the levels of supplementation in mice that produce beneficial effects are achievable in people, it appears that health benefits of NR will be translatable to humans safely.” This study was also featured on IowaNow.

Davies and Taylor awarded FOEDRC Pilot & Feasibility Grants

October 3rd, 2016 by Maren Rogers

Drs. Brandon Davies and Eric Taylor have been awarded FOEDRC Pilot & Feasiblity Grants of $50,000 to support their research proposal, with the possibility for a second year of funding, for a total of $100,000 over a two-year period.

Dr. Davies’s proposal entitled “Skeletal Muscle Programming of Capillary Endothelial Cells,” aims to to identify how skeletal muscle cells program adjacent endothelial cells to deliver triglyceride-derived fatty acids to muscle.

Dr. Taylor’s proposal entitled “A novel regulator of glutamine-driven gluconeogenesis,” aims to determine the biochemical activity of a recently discovered  poorly annotated protein that is important for using the amino acid glutamine for gluconeogenesis, the mechanisms underlying its role in glutamine-driven gluconeogenesis, and how its function contributes to hyperglycemia during type-2 diabetes.

Grant Young Receives Ponsetti Award for Research in Orthopedics

September 16th, 2016 by Judy Means
Grant Young, former Biochemistry undergraduate major, received “The Ponsetti Award for Research in Orthopedics” for his summer research as a U of Iowa medical student.

Highlights of the 7th Annual Biochemistry Retreat

September 12th, 2016 by Briana Horwath

The Department of Biochemistry held their 7th Annual Retreat on August 20, 2016, in the Coralville Public Library. The retreat featured 5 faculty talks and 34 poster presentations.

It was exciting to learn about all of the science happening in the Department! Congratulations to the winners of the various contests listed below.

Graduate Student Poster Competition:
1st Place: Lalita Oonthonpan (Taylor Lab) “Elucidating structure-function relationship of the mitochondrial pyruvate carrier”
2nd Place: Will Hacker (Elcock Lab) “Modeling the E. coli nucleoid subject to experimental restraints”
3rd Place: Mark Miller (Elcock Lab) “Re-parameterization of protein force fields guided by osmotic coefficient measurements from molecular dynamics simulations”

Postdoctoral/Medical Fellows and Research Staff Poster Competition:
Shyamal Subramanyam, Postdoc (M. Spies Lab) “Tyrosine phosphyorylation stimulates activity of human RAD51 recombinase through altered nucleoprotein filament dynamcs”

Undergraduate Poster Competition:
Rick Young (Wallrath Lab) “DNA damage associated with muscular dystrophy”

X-Scientist Theme Competition:
Sarah Hengel, aka Aqua Structure Girl (ASG) with her superpowers to shrink herself to the size of a water molecule, jump into protein solutions, and with her eyes shoot X-rays at the protein of interest for structural determination

2016 IDT & Smith-Gehring Graduate Fellowships

August 25th, 2016 by Maren Rogers

The IDT and Smith-Gehring Graduate Fellowships are awarded to three of the most meritorious second year Biochemistry graduate students based on academic and research achievements.

The 2016 IDT Graduate Fellows are Colleen Caldwell and Timothy Collingsworth:

Caldwell, Colleen

Ms. Colleen Caldwell performed extremely well in classes during her first year in the graduate program.  Work she did during her rotations is likely to earn her a contribution to research papers from two labs. Ms. Caldwell graduated cum laude with a bachelor’s degree in Biochemistry and Molecular Biology and a minor in Neuroscience from the Gustavus Adolphus College in St. Peter, MN in spring 2015. She had a brilliant undergraduate career and her application to our graduate program definitely stood out. In the laboratory of Dr. Maria Spies, she is working on a project focused on deciphering the molecular mechanism of human DNA helicase RTEL1 (regulator of telomere length). Defects in the RTEL1 helicase are associated with a broad spectrum of human diseases ranging from cancer to Crohn’s. Ms. Caldwell plans to take a full advantage of Dr. Spies’ lab expertise in DNA repair helicases and custom built single-molecule equipment to decipher the RTEL1 mechanism and to gain insights into its physiological roles outside of the telomeres. In collaboration with the X-ray crystallography core and Dr. M. Todd Washington’s lab, Ms. Caldwell will also add a structural biology component to her work on RTEL1 in definition of the association between RTEL1 helicase and the PCNA processivity clamp that integrates RTEL1 activity into cellular processes that ensure accurate replication.

Collingsworth, Tim

Mr. Timothy Collingsworth also had an exemplary first year in the graduate program. In addition, Mr. Collingsworth’s overall positive attitude and enthusiasm stood out to us. Mr. Collingsworth, who grew up in Cedar Rapids, graduated with a bachelor’s degree in Biochemistry and minors in Computer Science and Spanish from the University of Iowa in spring 2015. Mr. Collingsworth is training in the laboratory of Dr. Michael Schnieders and is working on a project in collaboration with Dr. Michael Welsh aimed to develop computational tools to combat cystic fibrosis (CF). CF is caused by mutations in the gene that encodes the cystic fibrosis transmembrane conductance regulator (CFTR) anion channel. In humans and pigs lacking CFTR, unchecked H+ secretion by the nongastric H+/K+ adenosine triphosphatase (ATP12A) acidifies airway surface liquid, while mice that lack CFTR express little ATP12A and secrete minimal H+. Thus, airway surface liquid in CF and non-CF mice have similar pH, suggesting that inhibiting ATP12A can reverse host defense abnormalities and treat CF in humans. The goal of Mr. Collingsworth’s project is to use computer aided molecular design to create specific inhibitors of ATP12A function.

The 2016 Smith-Gehring Graduate Fellow is Hannah Miller:

Miller, Hannah Headshot

Ms. Hannah Miller performed extremely well in classes during her first year in the graduate program. Ms. Miller is the department’s first Fast Track PhD student. The Fast Track program allows for high achieving University of Iowa undergraduate students to take PhD course work during their final undergraduate year. To qualify for this program Ms. Miller participated in an extensive amount of formal research in Dr. Todd Washington’s lab and maintained an exemplary academic record. Ms. Miller stated, “I was interested in the Fast Track PhD program in Biochemistry because it is a unique opportunity to dive deeper into research at a young age. The program gives me the opportunity to join a lab with my first year, giving me a great start on my thesis project. I’m very excited to see how the program will challenge me and allow me to progress as a scientist.” Ms. Miller has joined the laboratory of Dr. Kris DeMali. She is currently working on a project aimed at understanding how cells sense and transmit externally applied forces and dissecting how this process becomes dysregulated during tumorigenesis.

Congratulations to the 2016 IDT & Smith-Gehring Graduate Fellows!

John Donelson receives 2016 Distinguished Mentor Award

August 25th, 2016 by Maren Rogers

donelson 200x300Dr. John Donelson, Professor Emeritus and former Head of Biochemistry, has been awarded the 2016 Distinguished Mentor Award. Professor Donelson has an outstanding record of mentoring trainees, faculty and staff at all levels. By his example, and with his guidance and leadership, he has influenced the lives and careers of many scientists and physicians at Iowa and beyond.

After obtaining a BS in Biophysics from Iowa State, he left the comforts of his youth by joining the American Peace Corps. He taught math, chemistry and physics in Ghana, West Africa. There, he saw firsthand the havoc wrought by infectious diseases. This motivated him to return to the US, where he attended graduate school at Cornell University. He earned his PhD in Biochemistry in 1971 for work on exonucleolytic DNA sequence determination with DNA polymerase I. He was  awarded a Helen Hay Whitney fellowship to further develop DNA sequencing and phage molecular biology in Cambridge, England, with Nobel Laureate Fred Sanger.  Indeed, work that John and other luminaries did with Sanger during this period contributed to Sanger’s second Nobel Prize for dideoxy sequencing of DNA. Professor Donelson continued pioneering studies in molecular biology during a brief second postdoc at Stanford University with Professor David Hogness. He was then recruited to the Department of Biochemistry at the University of Iowa as an Assistant Professor in 1974. He brought back to Iowa his worldly experiences and cutting edge molecular techniques that fueled his research program on African trypanosomes for the next 37 years.

Among his 255 publications are landmark papers such as a 1974 Cell paper with Pieter Weinsick and David Hogness on chromosome mapping in the fruit fly Drosophila, his 1980 Nature paper reporting the sequence of the yeast 2 micron plasmid, and his groundbreaking contributions to the genomics of trypanosomes in three Nature and Science papers. Twenty years after first reading Scientific American articles in Africa, he wrote a 1985 review article in the same journal on “How the African Trypanosome Changes Its Coat.”

Professor Donelson served as a research mentor of 29 PhD students and 23 postdoctoral fellows. Bruce Citron, Director, Laboratory of Molecular Biology, Professor of Molecular Medicine, USF College of Medicine states “He truly cared about his students and the graduate program and provided just the right amount of guidance – not too much and not too little.” Kent Hill, Professor, Department of Microbiology, Immunology and Molecular Genetics, UCLA reports that Professor Donelson was “always available for discussion and continuously works to identify opportunities for enriching the training experience of the people in his lab or classroom.” Dr. Shiyong Li, Associate Professor, Emory University School of Medicine, states “Professor Donelson had a remarkable ability to phrase constructive criticism in a way that was encouraging, leaving me wanting to work harder and better”. Nearly all of his trainees have gone on to prominent positions in academia and industry; many are leaders in molecular parasitology and molecular biology, thereby carrying on his legacy.

At the time of his University of Iowa retirement in 2011, Dr. Louis Miller of the National Institutes of Allergy and Infectious Diseases wrote a commendation to Dr. Donelson stating, “you were always the leader in the world in molecular biology of Trypanosomes.” In 2012, John surprised us by being offered and accepting an appointment as a visiting professor at the Federal University of Rio Grand do Norte in Brazil, where he teaches biochemistry and conducts genomic research on leishmania.

Dr. Donelson will be honored at the 2016 Distinguished Mentor Award Celebration and Lecture from 3 to 5 p.m., Thursday, Sept. 1, in 1110A MERF.

Elizabeth Boehm and Zhen Xu Earn PhD Summer 2016

August 24th, 2016 by Briana Horwath

Boehm, ElizabethElizabeth Boehm, a Biochemistry PhD student mentored by Dr. Todd Washington, received her PhD on August 5, 2016. Elizabeth’s thesis is entitled, ” The regulation of translesion synthesis through the binding and activation of polymerases by PCNA”. Elizabeth has accepted a position as a postdoctoral position at Harvard Medical School in Johannes Walter’s lab to study DNA replication and repair in Xenopus laevis.


Xu, Zhen

Zhen Xu, a Biochemistry PhD student mentored by Dr. Ernesto Fuentes, received his PhD on August 5, 2016. Zhen’s thesis is entitled, “Auto-inhibition mechanism of the guanine nucleotide exchange factor TIAM1.” Zhen has accepted a postdoctoral position at the University of Iowa in Ernesto Fuentes’ lab.

Congrats Elizabeth and Zhen!

Maria Spies lab publishes in eLife Sciences

August 22nd, 2016 by Maren Rogers

Dr. Maria Spies laboratory in collaboration with Dr. Ashley Spies laboratory recently published an eLife Sciences article entitled “Small-molecule inhibitors identify the RAD52-ssDNA interaction as critical for recovery from replication stress and for survival of BRCA2 deficient cells.” In this study, Hengel et al. developed a high throughput biophysical method to search through a large collection of small molecules to find those that prevent RAD52 from binding to DNA and then used the information about how the small molecules bind to RAD52 to preform further computational screening. This identified a natural compound that competes with single-stranded DNA to bind to RAD52. The activity of this molecule was then validated using biophysical methods. The methods used by Hengel et al. provide the foundation for further searches for new anticancer drugs. Future studies that employ the small molecule drugs identified so far will also help to determine exactly how RAD52 works in human cells and how it helps cancer cells to survive.

This article was Ms. Sarah Hengel’s first, first author paper. Congratulations, Sarah!

Baker awarded R21 from National Eye Institute

August 22nd, 2016 by Maren Rogers

Drs. Sheila Baker and Amy Lee (Molecular Physiology & Biophysics) in collaboration with Arlene Drack (Ophthalmology & Visual Sciences) have been awarded an R21 grant from the National Eye Institute entitled “Rescue of photoreceptor synapses”.  This multidisciplinary team will explore the regenerative capacity of photoreceptor synapses and develop a method for making novel retinal gene therapy vectors in an effort to create new treatments for blindness.